Antimicrobial Activities and Influential Factors of Temporin Family Antimicrobial Peptides Derived from Hylarana guentheri of Hainan

In our report, the chemical synthesis method were performed to synthesis four antimicrobial peptides, temporin-GHa，temporin-GHb，temporin-GHc, and temporin-GHd, their antimicrobial activities were determined, and the effects of temperature, heat-treated time, and pH value on antimicrobial activities were analyzed. The results indicated that temporin-GHa～GHd has the antimicrobial activities against Gram-positive strains, Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Gram-negative strains Escherichia coli (D 31), and fungus, Monilia albican; S. aureus was the most sensitive strains, and the minimum inhibitory concentration (MIC) of four temporin family antimicrobial peptides against S. aureus is 6.8, 6.8, 12.9, and 12.7 μM, respectively; temporin GHa，temporin GHc和temporin GHd were stable over a broad range of temperatures (25-100 ℃), and pH (2-10).