Effects of Inducing Methods on Production of（6-methysalicylic acid） in Saccharomyces（S.cerevisiae）

Polyketides(PKS) is a group of secondary metabolites, exhibiting remarkable diversity both in terms of their structure and function. Many kinds of them have been developed into natural products that are known to possess a wealth of pharmacologically important activities, including antimicrobial, antifungal, and anti-tumor properties. Recently, 6-methylsalicylic acid synthase(6-MSAS) of Penicillium patulum is the sole example of a fungal PKS that has been characterized at both the enzyme and gene levels. In this project, effects of different inducing methods on production of 6-MSA in Saccharomyces(S.cerevisiae334) were analyzed. The results show that: there is significant difference between different inducing times except for 24h and 48h, and the highest production was obtained by inducing at 24h(104.51mg•L^-1). In additional, there are significant difference between different amounts of inducer also, and the highest production was obtained in the medium containing 10% galactose(121.61mg•L^-1). Different kind inducing methods will affect production of 6-MSA in S. cerevisiae significantly.