Abstract: Cadmium （Cd） pollution is a serious issue for agriculture and human health， however， until now， no highly efficient methods for Cd bioremediation were successfully developed. In our previous study， an Enterobacter cloacae strain Cu6 with multi heavy metal （including Cd） resistance， which cannot be used for soil Cd remediation due to its low Cd resistance， was isolated. In the report， we obtained another strain （LPY6） derived from Cu6 by the long-term adaptive evolution method. In liquid media， LPY6 can resist to 5 000 mg·L^-1 （22 mmol·L^-1） CdCl₂·2.5H₂O， which is higher than any reported Cd-resistant bacteria. Besides the Cd resistance， LPY6 can also resist other heavy metals including Zn， Mn， Cu， Co， and Fe. LPY6 can alleviate the germination and growth inhibition of Cd on Brassica oleracea in plate and pot assays. In conclusion， a high Cd resistance bacterium strain that has application potential in soil Cd bioremediation is obtained.